BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing

Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time....

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Detalhes bibliográficos
Publicado no:Genome Biol
Main Authors: Alpern, Daniel, Gardeux, Vincent, Russeil, Julie, Mangeat, Bastien, Meireles-Filho, Antonio C. A., Breysse, Romane, Hacker, David, Deplancke, Bart
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2019
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6474054/
https://ncbi.nlm.nih.gov/pubmed/30999927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-019-1671-x
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