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Expanding the Dynamic Range of Fluorescence Assays through Single-Molecule Counting and Intensity Calibration
Surface capture assays can measure fluorescently labeled analytes across a 1,000-fold concentration range and at the sub-nanomolar level, but many biological molecules exhibit 1,000,000-fold variations in abundance down to the femtomolar level. The goal of this work is to expand the dynamic range of...
Gardado en:
| Publicado en: | J Am Chem Soc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2018
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6462413/ https://ncbi.nlm.nih.gov/pubmed/30215524 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.8b08879 |
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