A Rapid Method for Detection of Salmonella in Milk Based on Extraction of mRNA Using Magnetic Capture Probes and RT-qPCR

Magnetic separation is an efficient method for target enrichment and elimination of inhibitors in the molecular detection systems for foodborne pathogens. In this study, we prepared magnetic capture probes by modifying oligonucleotides complementary to target sequences on the surface of amino-modifi...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Bai, Yalong, Cui, Yan, Suo, Yujuan, Shi, Chunlei, Wang, Dapeng, Shi, Xianming
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2019
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6459957/
https://ncbi.nlm.nih.gov/pubmed/31024515
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2019.00770
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