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Three-Color Single-Molecule FRET and Fluorescence Lifetime Analysis of Fast Protein Folding

We describe the theory, experiment, and analysis of three-color Förster resonance energy transfer (FRET) spectroscopy for probing conformational dynamics of a fast-folding protein, α(3)D. In three-color FRET, site-specific labeling of fluorophores is required to avoid ambiguity resulting from variou...

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Publicado en:J Phys Chem B
Main Authors: Yoo, Janghyun, Louis, John M., Gopich, Irina V., Chung, Hoi Sung
Formato: Artigo
Idioma:Inglês
Publicado: 2018
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6458097/
https://ncbi.nlm.nih.gov/pubmed/30230835
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jpcb.8b07768
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