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Oligomerization of the tetramerization domain of p53 probed by two- and three-color single-molecule FRET

We describe a method that combines two- and three-color single-molecule FRET spectroscopy with 2D FRET efficiency–lifetime analysis to probe the oligomerization process of intrinsically disordered proteins. This method is applied to the oligomerization of the tetramerization domain (TD) of the tumor...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Chung, Hoi Sung, Meng, Fanjie, Kim, Jae-Yeol, McHale, Kevin, Gopich, Irina V., Louis, John M.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5565410/
https://ncbi.nlm.nih.gov/pubmed/28760960
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1700357114
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