Oligomerization of the tetramerization domain of p53 probed by two- and three-color single-molecule FRET

We describe a method that combines two- and three-color single-molecule FRET spectroscopy with 2D FRET efficiency–lifetime analysis to probe the oligomerization process of intrinsically disordered proteins. This method is applied to the oligomerization of the tetramerization domain (TD) of the tumor...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Chung, Hoi Sung, Meng, Fanjie, Kim, Jae-Yeol, McHale, Kevin, Gopich, Irina V., Louis, John M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2017
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PNAS Plus
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5565410/
https://ncbi.nlm.nih.gov/pubmed/28760960
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1700357114
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