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The Use of Oligonucleotide Recombination to Generate Isogenic Mutants of Clinical Isolates of Pseudomonas aeruginosa

Oligonucleotide recombination allows the introduction of specific point mutations into the bacterial genome. Here we report the successful application of this technique to clinical isolates of Pseudomonas aeruginosa to allow subsequent investigations of the biological impact associated with resistan...

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書目詳細資料
發表在:J Microbiol Methods
Main Authors: Agnello, Melissa, Wong-Beringer, Annie
格式: Artigo
語言:Inglês
出版: 2013
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC6453815/
https://ncbi.nlm.nih.gov/pubmed/24374216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2013.11.014
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