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The Use of Oligonucleotide Recombination to Generate Isogenic Mutants of Clinical Isolates of Pseudomonas aeruginosa
Oligonucleotide recombination allows the introduction of specific point mutations into the bacterial genome. Here we report the successful application of this technique to clinical isolates of Pseudomonas aeruginosa to allow subsequent investigations of the biological impact associated with resistan...
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| Publicado no: | J Microbiol Methods |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6453815/ https://ncbi.nlm.nih.gov/pubmed/24374216 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2013.11.014 |
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