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Preparation of RNA 3’ end sequencing libraries of total and 4-thiouracillabeled RNA for Simultaneous Measurement of Transcription, RNA Synthesis and Decay in S. cerevisiae

Cellular RNA levels are determined by the rates of RNA transcription from the gene template and subsequent RNA stability. Knowledge about both transcription and RNA decay is, therefore, necessary to interpret RNA levels and gene expression, especially during cellular processes where these parameters...

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Vydáno v:Bio Protoc
Hlavní autoři: Schmid, Manfred, Tudek, Agnieszka, Jensen, Torben Heick
Médium: Artigo
Jazyk:Inglês
Vydáno: Bio-Protocol 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6436697/
https://ncbi.nlm.nih.gov/pubmed/30931349
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3189
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