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Preparation of RNA 3’ end sequencing libraries of total and 4-thiouracillabeled RNA for Simultaneous Measurement of Transcription, RNA Synthesis and Decay in S. cerevisiae
Cellular RNA levels are determined by the rates of RNA transcription from the gene template and subsequent RNA stability. Knowledge about both transcription and RNA decay is, therefore, necessary to interpret RNA levels and gene expression, especially during cellular processes where these parameters...
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| Veröffentlicht in: | Bio Protoc |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Bio-Protocol
2019
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6436697/ https://ncbi.nlm.nih.gov/pubmed/30931349 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3189 |
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