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Selection-free markerless genome manipulations in the polyploid bacterium Thermus thermophilus
A genome manipulation approach based on double-crossover homologous recombination was developed in the polyploid model organism Thermus thermophilus HB27 without the use of any selectable marker. The method was established and optimized by targeting the megaplasmid-encoded β-glucosidase gene bgl. Wh...
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| Published in: | 3 Biotech |
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| Main Author: | |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Springer International Publishing
2019
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6431304/ https://ncbi.nlm.nih.gov/pubmed/30944795 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-019-1682-z |
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