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Selection-free markerless genome manipulations in the polyploid bacterium Thermus thermophilus

A genome manipulation approach based on double-crossover homologous recombination was developed in the polyploid model organism Thermus thermophilus HB27 without the use of any selectable marker. The method was established and optimized by targeting the megaplasmid-encoded β-glucosidase gene bgl. Wh...

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Publicado en:3 Biotech
Autor principal: Li, Haijuan
Formato: Artigo
Lenguaje:Inglês
Publicado: Springer International Publishing 2019
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6431304/
https://ncbi.nlm.nih.gov/pubmed/30944795
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-019-1682-z
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