CRISPR Interference for Rapid Knockdown of Essential Cell Cycle Genes in Lactobacillus plantarum

Studies of essential genes in bacteria are often hampered by the lack of accessible genetic tools. This is also the case for Lactobacillus plantarum, a key species in food and health applications. Here, we develop a clustered regularly interspaced short palindromic repeat interference (CRISPRi) syst...

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Pubblicato in:mSphere
Autori principali: Myrbråten, Ine Storaker, Wiull, Kamilla, Salehian, Zhian, Håvarstein, Leiv Sigve, Straume, Daniel, Mathiesen, Geir, Kjos, Morten
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2019
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6429040/
https://ncbi.nlm.nih.gov/pubmed/30894429
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.00007-19
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