Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

BACKGROUND: Transgenic zebrafish lines with the expression of a fluorescent reporter under the control of a cell-type specific promoter, enable transcriptome analysis of FACS sorted cell populations. RNA quality and yield are key determinant factors for accurate expression profiling. Limited cell nu...

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Detalhes bibliográficos
Publicado no:BMC Genomics
Main Authors: Loontiens, Siebe, Depestel, Lisa, Vanhauwaert, Suzanne, Dewyn, Givani, Gistelinck, Charlotte, Verboom, Karen, Van Loocke, Wouter, Matthijssens, Filip, Willaert, Andy, Vandesompele, Jo, Speleman, Frank, Durinck, Kaat
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2019
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6425699/
https://ncbi.nlm.nih.gov/pubmed/30894119
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-019-5608-2
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