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Protein oligomerization and mobility within the nuclear envelope evaluated by the time-shifted mean-segmented Q factor

Analysis of fluorescence fluctuation experiments by the mean-segmented Q (MSQ) method was recently used to successfully characterize the oligomeric state and mobility of proteins within the nuclear envelope (NE) of living cells. However, two significant shortcomings of MSQ were recognized. Non-ideal...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Methods
Hauptverfasser: Hennen, Jared, Hur, Kwang-Ho, Karuka, Siddarth Reddy, Gant Luxton, G.W., Mueller, Joachim D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6401277/
https://ncbi.nlm.nih.gov/pubmed/30268407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2018.09.008
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