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Protein oligomerization and mobility within the nuclear envelope evaluated by the time-shifted mean-segmented Q factor

Analysis of fluorescence fluctuation experiments by the mean-segmented Q (MSQ) method was recently used to successfully characterize the oligomeric state and mobility of proteins within the nuclear envelope (NE) of living cells. However, two significant shortcomings of MSQ were recognized. Non-ideal...

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Detalhes bibliográficos
Publicado no:Methods
Main Authors: Hennen, Jared, Hur, Kwang-Ho, Karuka, Siddarth Reddy, Gant Luxton, G.W., Mueller, Joachim D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6401277/
https://ncbi.nlm.nih.gov/pubmed/30268407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2018.09.008
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