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Light-Controlled Affinity Purification of Protein Complexes Exemplified by the Resting ZAP70 Interactome

Multiprotein complexes control the behavior of cells, such as of lymphocytes of the immune system. Methods to affinity purify protein complexes and to determine their interactome by mass spectrometry are thus widely used. One drawback of these methods is the presence of false positives. In fact, the...

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Detalhes bibliográficos
Publicado no:Front Immunol
Main Authors: Hörner, Maximilian, Eble, Julian, Yousefi, O. Sascha, Schwarz, Jennifer, Warscheid, Bettina, Weber, Wilfried, Schamel, Wolfgang W. A.
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6399385/
https://ncbi.nlm.nih.gov/pubmed/30863395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fimmu.2019.00226
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