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Duplication and transformation of the Schizosaccharomyces pombe collection of deletion strains
We present an efficient and organized method of lithium acetate and polyethylene glycol-based transformation of plasmid DNA into the commercially available collection of Schizosaccharomyces pombe with single gene deletions. We also describe how to prepare a duplicate collection of the deletion strai...
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| Publicat a: | Methods Mol Biol |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2018
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6390284/ https://ncbi.nlm.nih.gov/pubmed/29423859 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7546-4_18 |
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