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High-frequency lithium acetate transformation of Schizosaccharomyces pombe
The introduction of ectopic DNA, such as plasmids, into yeast cells has for decades been a critical protocol for the study of this eukaryotic model system. We describe here an efficient transformation procedure for use in the fission yeast Schizosaccharomyces pombe. This method relies on chemical ag...
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| Publicado no: | Methods Mol Biol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6388634/ https://ncbi.nlm.nih.gov/pubmed/29423856 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7546-4_15 |
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