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High-frequency lithium acetate transformation of Schizosaccharomyces pombe

The introduction of ectopic DNA, such as plasmids, into yeast cells has for decades been a critical protocol for the study of this eukaryotic model system. We describe here an efficient transformation procedure for use in the fission yeast Schizosaccharomyces pombe. This method relies on chemical ag...

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Bibliografiska uppgifter
I publikationen:Methods Mol Biol
Huvudupphovsmän: Rai, Sudhir Kumar, Atwood-Moore, Angela, Levin, Henry L
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2018
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC6388634/
https://ncbi.nlm.nih.gov/pubmed/29423856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7546-4_15
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