Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice

The CRISPR/Cas9 system can efficiently introduce biallelic mutations in ES cells (ESCs), and its application with fluorescently-tagged ESCs enables phenotype analysis in chimeric mice. We have utilized ESCs that express EGFP in the cytosol and acrosome [EGR-G101 129S2 × (CAG/Acr-EGFP) B6] in previou...

Полное описание

Сохранить в:
Библиографические подробности
Опубликовано в: :Exp Anim
Главные авторы: Oura, Seiya, Miyata, Haruhiko, Noda, Taichi, Shimada, Keisuke, Matsumura, Takafumi, Morohoshi, Akane, Isotani, Ayako, Ikawa, Masahito
Формат: Artigo
Язык:Inglês
Опубликовано: Japanese Association for Laboratory Animal Science 2018
Предметы:
Original
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC6389518/
https://ncbi.nlm.nih.gov/pubmed/30089752
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1538/expanim.18-0071
Метки: Добавить метку
Нет меток, Требуется 1-ая метка записи!

Схожие документы