Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice

The CRISPR/Cas9 system can efficiently introduce biallelic mutations in ES cells (ESCs), and its application with fluorescently-tagged ESCs enables phenotype analysis in chimeric mice. We have utilized ESCs that express EGFP in the cytosol and acrosome [EGR-G101 129S2 × (CAG/Acr-EGFP) B6] in previou...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Exp Anim
Prif Awduron: Oura, Seiya, Miyata, Haruhiko, Noda, Taichi, Shimada, Keisuke, Matsumura, Takafumi, Morohoshi, Akane, Isotani, Ayako, Ikawa, Masahito
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Japanese Association for Laboratory Animal Science 2018
Pynciau:
Original
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC6389518/
https://ncbi.nlm.nih.gov/pubmed/30089752
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1538/expanim.18-0071
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