Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice

The CRISPR/Cas9 system can efficiently introduce biallelic mutations in ES cells (ESCs), and its application with fluorescently-tagged ESCs enables phenotype analysis in chimeric mice. We have utilized ESCs that express EGFP in the cytosol and acrosome [EGR-G101 129S2 × (CAG/Acr-EGFP) B6] in previou...

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Pubblicato in:Exp Anim
Autori principali: Oura, Seiya, Miyata, Haruhiko, Noda, Taichi, Shimada, Keisuke, Matsumura, Takafumi, Morohoshi, Akane, Isotani, Ayako, Ikawa, Masahito
Natura: Artigo
Lingua:Inglês
Pubblicazione: Japanese Association for Laboratory Animal Science 2018
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6389518/
https://ncbi.nlm.nih.gov/pubmed/30089752
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1538/expanim.18-0071
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