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CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice
Targeted gene disrupted mice can be efficiently generated by expressing a single guide RNA (sgRNA)/CAS9 complex in the zygote. However, the limited success of complicated genome editing, such as large deletions, point mutations, and knockins, remains to be improved. Further, the mosaicism in founder...
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| Publicado no: | Sci Rep |
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| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4987700/ https://ncbi.nlm.nih.gov/pubmed/27530713 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep31666 |
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