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Analysis of RNA 5′ ends: phosphate enumeration and cap characterization

The function and fate of cellular RNAs are often governed by the phosphorylation state at the 5′ end or the identity of whatever cap may be present there. Here we describe methods for examining these important 5′-terminal features on any cellular or synthetic RNA of interest that can be detected by...

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Bibliografske podrobnosti
izdano v:Methods
Main Authors: Luciano, Daniel J., Belasco, Joel G.
Format: Artigo
Jezik:Inglês
Izdano: 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6387834/
https://ncbi.nlm.nih.gov/pubmed/30419334
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2018.10.023
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