Cell-Free Protein Synthesis Using S30 Extracts from Escherichia coli RFzero Strains for Efficient Incorporation of Non-Natural Amino Acids into Proteins

Cell-free protein synthesis is useful for synthesizing difficult targets. The site-specific incorporation of non-natural amino acids into proteins is a powerful protein engineering method. In this study, we optimized the protocol for cell extract preparation from the Escherichia coli strain RFzero-i...

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Detalhes bibliográficos
Publicado no:Int J Mol Sci
Main Authors: Adachi, Jiro, Katsura, Kazushige, Seki, Eiko, Takemoto, Chie, Shirouzu, Mikako, Terada, Takaho, Mukai, Takahito, Sakamoto, Kensaku, Yokoyama, Shigeyuki
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6387211/
https://ncbi.nlm.nih.gov/pubmed/30678326
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms20030492
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