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Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen

Characterizing complex viral transcriptomes by conventional RNA sequencing approaches is complicated by high gene density, overlapping reading frames, and complex splicing patterns. Direct RNA sequencing (direct RNA-seq) using nanopore arrays offers an exciting alternative whereby individual polyade...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Depledge, Daniel P., Srinivas, Kalanghad Puthankalam, Sadaoka, Tomohiko, Bready, Devin, Mori, Yasuko, Placantonakis, Dimitris G., Mohr, Ian, Wilson, Angus C.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6376126/
https://ncbi.nlm.nih.gov/pubmed/30765700
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-08734-9
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