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Best Practices for Preparing a Single Cell Suspension from Solid Tissues for Flow Cytometry
Preparing a single cell suspension is a critical step in any solid tissue flow cytometry experiment. Tissue dissection, enzymatic digestion, and mechanical dissociation are three significant steps leading to the degradation of the extracellular matrix and the isolation of single cells, allowing the...
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| Veröffentlicht in: | Cytometry A |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6375754/ https://ncbi.nlm.nih.gov/pubmed/30523671 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cyto.a.23690 |
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