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Best Practices for Preparing a Single Cell Suspension from Solid Tissues for Flow Cytometry

Preparing a single cell suspension is a critical step in any solid tissue flow cytometry experiment. Tissue dissection, enzymatic digestion, and mechanical dissociation are three significant steps leading to the degradation of the extracellular matrix and the isolation of single cells, allowing the...

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Detalhes bibliográficos
Publicado no:Cytometry A
Main Authors: Reichard, Andrew, Asosingh, Kewal
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6375754/
https://ncbi.nlm.nih.gov/pubmed/30523671
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cyto.a.23690
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