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Generation of TGFBI knockout ABCG2+/ABCB5+ double-positive limbal epithelial stem cells by CRISPR/Cas9-mediated genome editing

Corneal dystrophy is an autosomal dominant disorder caused by mutations of the transforming growth factor β-induced (TGFBI) gene on chromosome 5q31.8. This disease is therefore ideally suited for gene therapy using genome-editing technology. Here, we isolated human limbal epithelial stem cells (ABCG...

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Veröffentlicht in:PLoS One
Hauptverfasser: Kim, Eung Kweon, Kim, Seunghyuk, Maeng, Yong-Sun
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2019
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6372159/
https://ncbi.nlm.nih.gov/pubmed/30753226
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0211864
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