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Transcriptome-based identification and validation of reference genes for plant-bacteria interaction studies using Nicotiana benthamiana

RT-qPCR is a widely used technique for the analysis of gene expression. Accurate estimation of transcript abundance relies strongly on a normalization that requires the use of reference genes that are stably expressed in the conditions analyzed. Initially, they were adopted from those used in Northe...

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Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Pombo, Marina A., Ramos, Romina N., Zheng, Yi, Fei, Zhangjun, Martin, Gregory B., Rosli, Hernan G.
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6367355/
https://ncbi.nlm.nih.gov/pubmed/30733563
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-38247-2
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