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Determining Cholesterol Binding to Membrane Proteins by Cholesterol (13)C Labeling in Yeast and Dynamic Nuclear Polarization NMR
We present a general strategy for determining the cholesterol-binding site of eukaryotic membrane proteins in native-like lipid membranes by NMR spectroscopy. The strategy combines yeast biosynthetic (13)C enrichment of cholesterol with detection of protein-cholesterol (13)C-(13)C cross peaks in 2D...
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| Veröffentlicht in: | J Am Chem Soc |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6361393/ https://ncbi.nlm.nih.gov/pubmed/30338997 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.8b09658 |
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