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Imaging Cell-Type-Specific Dynamics of mRNAs in Living Mouse Brain
We describe a method for visualizing mRNAs in living mouse. Nascent transcripts and cytoplasmic mRNAs were labeled via lentiviral expression of MS2 coat protein (MCP) tagged with fluorescent protein (MCP-XFP) in knock-in mice whose β-actin mRNAs contained MCP binding stem loops (MBS). Then the mRNA...
Shranjeno v:
| izdano v: | Methods |
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| Main Authors: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2018
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6351228/ https://ncbi.nlm.nih.gov/pubmed/30067943 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2018.07.009 |
| Oznake: |
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