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Background free imaging of single mRNAs in live cells using split fluorescent proteins
We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP t...
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| Hlavní autoři: | , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Nature Publishing Group
2014
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3885884/ https://ncbi.nlm.nih.gov/pubmed/24402470 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep03615 |
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