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Liposome-based transfection enhances RNAi and CRISPR-mediated mutagenesis in non-model nematode systems

Nematodes belong to one of the most diverse animal phyla. However, functional genomic studies in nematodes, other than in a few species, have often been limited in their reliability and success. Here we report that by combining liposome-based technology with microinjection, we were able to establish...

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Publicat a:Sci Rep
Autors principals: Adams, Sally, Pathak, Prachi, Shao, Hongguang, Lok, James B., Pires-daSilva, Andre
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6345965/
https://ncbi.nlm.nih.gov/pubmed/30679624
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-37036-1
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