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Charge State Coalescence During Electrospray Ionization Improves Peptide Identification by Tandem Mass Spectrometry

We report the effects of supercharging reagents dimethyl sulphoxide (DMSO) and m-nitrobenzyl alcohol (m-NBA) applied to untargeted peptide identification, with special emphasis on nontryptic peptides. Peptides generated from a mixture of five standard proteins digested with trypsin, elastase, or pep...

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Bibliographic Details
Published in:J Am Soc Mass Spectrom
Main Authors: Meyer, Jesse G., Komives, Elizabeth A.
Format: Artigo
Language:Inglês
Published: 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6345509/
https://ncbi.nlm.nih.gov/pubmed/22610994
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-012-0404-0
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