Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion

Complementation-dependent fluorescence is a powerful way to study co-localization or interactions between biomolecules. A split-GFP variant, involving the self-associating GFP 1–10 and GFP 11, has previously provided a convenient approach to measure recombinant protein titers in cell supernatants. A...

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Vydáno v:Sci Rep
Hlavní autoři: Lundqvist, Magnus, Thalén, Niklas, Volk, Anna-Luisa, Hansen, Henning Gram, von Otter, Eric, Nygren, Per-Åke, Uhlen, Mathias, Rockberg, Johan
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2019
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6342966/
https://ncbi.nlm.nih.gov/pubmed/30670736
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-36559-x
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