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Kinetic isotope effect studies on the de novo rate of chromophore formation in fast- and slow-maturing GFP variants
The maturation process of green fluorescent protein (GFP) entails a protein oxidation reaction triggered by spontaneous backbone condensation. The chromophore is generated by full conjugation of the Tyr66 phenolic group with the heterocycle, a process that requires C-H bond scission at the benzylic...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2008
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2643082/ https://ncbi.nlm.nih.gov/pubmed/18759496 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi8007164 |
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