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Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9

CRISPR-Cas9 is a powerful gene editing technique that can induce mutations in a target gene of interest in almost any mammalian cell line. However, its practicality can be limited if target cell lines are difficult to transfect and do not proliferate. In the current study, we have developed a stream...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Sci Rep
Päätekijät: Jin, Yuan-Hu, Joo, Hyunjeong, Lee, Kwangjun, Kim, Hyeongseok, Didier, Ruth, Yang, Young, Shin, Heungsop, Lee, Choogon
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Nature Publishing Group UK 2019
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC6342919/
https://ncbi.nlm.nih.gov/pubmed/30670765
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-36736-y
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