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Aptamer Conformation Switching-Induced Two-Stage Amplification for Fluorescent Detection of Proteins

Basing on the conformation change of aptamer caused by proteins, a simple and sensitive protein fluorescent assay strategy is proposed, which is assisted by the isothermal amplification reaction of polymerase and nicking endonuclease. In the presence of platelet-derived growth factor (PDGF-BB), the...

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Detalhes bibliográficos
Publicado no:Sensors (Basel)
Main Authors: Yu, Qiao, Zhai, Fenfen, Zhou, Hong, Wang, Zonghua
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6338969/
https://ncbi.nlm.nih.gov/pubmed/30587808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/s19010077
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