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PrimerROC: accurate condition-independent dimer prediction using ROC analysis

To-date systematic testing and comparison of the accuracy of available primer-dimer prediction software has never been conducted, due in part to a lack of tools able to measure the efficacy of Gibbs free energy (ΔG) calculations at predicting dimer formation in PCR. To address this we have developed...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Johnston, Andrew D., Lu, Jennifer, Ru, Ke-lin, Korbie, Darren, Trau, Matt
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6338771/
https://ncbi.nlm.nih.gov/pubmed/30659212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-36612-9
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