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Purification of Saccharomyces cerevsiae Homologous Recombination Proteins Dmc1 and Rdh54/Tid1 and a Fluorescent D-loop Assay
Budding yeast Dmc1 is a member of the RecA family of strand exchange proteins essential for homologous recombination (HR) during meiosis. Dmc1 mediates the steps of homology search and DNA strand exchange reactions that are central to HR. To achieve optimum activity, Dmc1 requires a number of access...
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| Veröffentlicht in: | Methods Enzymol |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6335025/ https://ncbi.nlm.nih.gov/pubmed/29458764 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2017.12.003 |
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