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Purification of Saccharomyces cerevsiae Homologous Recombination Proteins Dmc1 and Rdh54/Tid1 and a Fluorescent D-loop Assay

Budding yeast Dmc1 is a member of the RecA family of strand exchange proteins essential for homologous recombination (HR) during meiosis. Dmc1 mediates the steps of homology search and DNA strand exchange reactions that are central to HR. To achieve optimum activity, Dmc1 requires a number of access...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Main Authors: Chan, Yuen-Ling, Bishop, Douglas K.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6335025/
https://ncbi.nlm.nih.gov/pubmed/29458764
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2017.12.003
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