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Mutational Analysis of the Role of the Glucansucrase Gtf180-ΔN Active Site Residues in Product and Linkage Specificity with Lactose as Acceptor Substrate
[Image: see text] Glucansucrase Gtf180-ΔN from Lactobacillus reuteri uses lactose as acceptor substrate to synthesize five glucosylated lactose molecules (F1–F5) with a degree of polymerization (DP) of 3–4 (GL34) and with (α1→2)/(α1→3)/(α1→4) glycosidic linkages. Q1140/W1065/N1029 mutations signific...
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| Publicado no: | J Agric Food Chem |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Chemical
Society
2018
|
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6328278/ https://ncbi.nlm.nih.gov/pubmed/30396274 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jafc.8b04486 |
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