Fluorescent labeling of CRISPR/Cas9 RNP for gene knockout in HSPCs and iPSCs reveals an essential role for GADD45b in stress response

CRISPR/Cas9-mediated gene editing of stem cells and primary cell types has several limitations for clinical applications. The direct delivery of ribonucleoprotein (RNP) complexes consisting of Cas9 nuclease and guide RNA (gRNA) has improved DNA- and virus-free gene modifications, but it does not ena...

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Dades bibliogràfiques
Publicat a:Blood Adv
Autors principals: Nasri, Masoud, Mir, Perihan, Dannenmann, Benjamin, Amend, Diana, Skroblyn, Tessa, Xu, Yun, Schulze-Osthoff, Klaus, Klimiankou, Maksim, Welte, Karl, Skokowa, Julia
Format: Artigo
Idioma:Inglês
Publicat: American Society of Hematology 2019
Matèries:
Hematopoiesis and Stem Cells
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6325296/
https://ncbi.nlm.nih.gov/pubmed/30622144
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1182/bloodadvances.2017015511
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