Fluorescent labeling of CRISPR/Cas9 RNP for gene knockout in HSPCs and iPSCs reveals an essential role for GADD45b in stress response

CRISPR/Cas9-mediated gene editing of stem cells and primary cell types has several limitations for clinical applications. The direct delivery of ribonucleoprotein (RNP) complexes consisting of Cas9 nuclease and guide RNA (gRNA) has improved DNA- and virus-free gene modifications, but it does not ena...

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Detalhes bibliográficos
Publicado no:Blood Adv
Main Authors: Nasri, Masoud, Mir, Perihan, Dannenmann, Benjamin, Amend, Diana, Skroblyn, Tessa, Xu, Yun, Schulze-Osthoff, Klaus, Klimiankou, Maksim, Welte, Karl, Skokowa, Julia
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Hematology 2019
Assuntos:
Hematopoiesis and Stem Cells
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6325296/
https://ncbi.nlm.nih.gov/pubmed/30622144
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1182/bloodadvances.2017015511
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