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CRISPR-Cas9 In Situ engineering of subtilisin E in Bacillus subtilis

CRISPR-Cas systems have become widely used across all fields of biology as a genome engineering tool. With its recent demonstration in the Gram positive industrial workhorse Bacillus subtilis, this tool has become an attractive option for rapid, markerless strain engineering of industrial production...

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發表在:PLoS One
Main Authors: Price, Marcus A., Cruz, Rita, Baxter, Scott, Escalettes, Franck, Rosser, Susan J.
格式: Artigo
語言:Inglês
出版: Public Library of Science 2019
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC6322745/
https://ncbi.nlm.nih.gov/pubmed/30615645
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0210121
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