Bovine epididymal spermatozoa treatment for in vitro fertilization: Heparin accelerates fertilization and enables a reduction in coincubation time

This study aimed to establish a protocol for in vitro embryo production using epididymal sperm (EP). Samples were obtained from ejaculated sperm (EJ) and the epididymis of 7 Gir bulls. First, the effect of heparin (+) on the viability, longevity (Experiment 1) and fertilization rates (Experiment 2)...

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Bibliographic Details
Published in:PLoS One
Main Authors: Cunha, Andrielle T. M., Carvalho, José O., Guimarães, Ana L. S., Leme, Ligiane O., Caixeta, Felippe M., Viana, João H. M., Dode, Margot A. N.
Format: Artigo
Language:Inglês
Published: Public Library of Science 2019
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6322719/
https://ncbi.nlm.nih.gov/pubmed/30615639
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0209692
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