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Further Stabilization of Alcalase Immobilized on Glyoxyl Supports: Amination Plus Modification with Glutaraldehyde

Alcalase was immobilized on glyoxyl 4% CL agarose beads. This permitted to have Alcalase preparations with 50% activity retention versus Boc-l-alanine 4-nitrophenyl ester. However, the recovered activity versus casein was under 20% at 50 °C, as it may be expected from the most likely area of the pro...

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Detalhes bibliográficos
Publicado no:Molecules
Main Authors: Hussain, Fouzia, Arana-Peña, Sara, Morellon-Sterling, Roberto, Barbosa, Oveimar, Ait Braham, Sabrina, Kamal, Shagufta, Fernandez-Lafuente, Roberto
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6320783/
https://ncbi.nlm.nih.gov/pubmed/30513981
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules23123188
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