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Advantages of Single-Nucleus over Single-Cell RNA Sequencing of Adult Kidney: Rare Cell Types and Novel Cell States Revealed in Fibrosis

BACKGROUND: A challenge for single-cell genomic studies in kidney and other solid tissues is generating a high-quality single-cell suspension that contains rare or difficult-to-dissociate cell types and is free of both RNA degradation and artifactual transcriptional stress responses. METHODS: We com...

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Detalles Bibliográficos
Publicado en:J Am Soc Nephrol
Autores principales: Wu, Haojia, Kirita, Yuhei, Donnelly, Erinn L., Humphreys, Benjamin D.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society of Nephrology 2019
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6317600/
https://ncbi.nlm.nih.gov/pubmed/30510133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1681/ASN.2018090912
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