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Two-color total internal refection fluorescence microscopy of exocytosis in endocrine cells.
We describe a comprehensive method for imaging and analysis of local protein dynamics at single sites of exocytosis in living cultured endocrine cells. This method is well suited to quantitatively map the complex dynamics of individual molecules at single sites of vesicle fusion in live cells.
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| Veröffentlicht in: | Methods Mol Biol |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6298027/ https://ncbi.nlm.nih.gov/pubmed/28324608 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6810-7_11 |
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