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Single-shot super-resolution total internal reflection fluorescence microscopy

We combine instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM), improving the lateral spatial resolution of TIRFM to 115 +/− 13 nm without compromising speed and enabling imaging frame rates up to 100 Hz over hundreds of time points. We ap...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Guo, Min, Chandris, Panagiotis, Giannini, John Paul, Trexler, Adam J., Fischer, Robert, Chen, Jiji, Vishwasrao, Harshad D., Rey-Suarez, Ivan, Wu, Yicong, Wu, Xufeng, Waterman, Clare M., Patterson, George H., Upadhyaya, Arpita, Taraska, Justin, Shroff, Hari
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7470603/
https://ncbi.nlm.nih.gov/pubmed/29735999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-018-0004-4
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