Detection of Mycobacterium tuberculosis purified ESAT-6 (Rv3875) by magnetic bead-coupled gold nanoparticle-based immuno-PCR assay

PURPOSE: Immuno-PCR (I-PCR), an ultrasensitive method, combines the versatility of ELISA with the exponential amplification capacity of PCR. Coupling of detection antibodies with the reporter DNA is a critical step of I-PCR. Gold nanoparticles (GNPs) and magnetic beads (MBs) are relatively easy to a...

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Bibliografiske detaljer
Udgivet i:Int J Nanomedicine
Main Authors: Singh, Netrapal, Dahiya, Bhawna, Radhakrishnan, Venkatraman Srinivasan, Prasad, Tulika, Mehta, Promod K
Format: Artigo
Sprog:Inglês
Udgivet: Dove Medical Press 2018
Fag:
Original Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6296691/
https://ncbi.nlm.nih.gov/pubmed/30587975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2147/IJN.S181052
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