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Decomposition of time-dependent fluorescence signals reveals codon-specific kinetics of protein synthesis
During protein synthesis, the nascent peptide chain traverses the peptide exit tunnel of the ribosome. We monitor the co-translational movement of the nascent peptide using a fluorescent probe attached to the N-terminus of the nascent chain. Due to fluorophore quenching, the time-dependent fluoresce...
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| Опубликовано в: : | Nucleic Acids Res |
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| Главные авторы: | , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
2018
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6294494/ https://ncbi.nlm.nih.gov/pubmed/30107440 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky740 |
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