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A Single H1 Promoter Can Drive Both Guide RNA and Endonuclease Expression in the CRISPR-Cas9 System

The RNA-guided endonuclease Cas9 (CRISPR-Cas9) genome editing system has been widely used for biomedical research and holds great potential for therapeutic applications in eukaryotes. The conventional vector-based CRISPR-Cas9 delivery system requires two different RNA polymerase promoters for expres...

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Vydáno v:Mol Ther Nucleic Acids
Hlavní autoři: Gao, Zongliang, Herrera-Carrillo, Elena, Berkhout, Ben
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society of Gene & Cell Therapy 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6288460/
https://ncbi.nlm.nih.gov/pubmed/30530211
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtn.2018.10.016
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